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Establishing
the purity of an essential oil
is of paramount importance
when it is to be
used in aromatherapy to treat physical or emotional conditions. At best,
essential oils that have been adulterated or
extended in any way will simply not deliver the health benefits that you
expect. At worst, there is the risk of serious skin irritation or sensitisation due to
an adverse reaction to the synthetic chemicals that have
been used to adulterate the essential oil.
Therefore, essential oils are
never purchased in bulk by Quinessence until they been subjected to the most rigorous
qualitative and quantitative analytical procedures to confirm their
purity and authenticity. The practice of oil adulteration has now
reached a very high level of sophistication, therefore a wide range
meticulous checks must be made to ensure that the purity of every single
essential oil has been confirmed.
Qualitative and
Quantitative Analysis
So what exactly what is the difference
between qualitative and quantitative analysis? Put simply,
qualitative testing helps to determine exactly what
individual constituents are present in an essential
oil, and quantitative testing provides
information on how
much
of each component is present.
In the hands of experts, this
information can be used to verify that the oil is indeed what it claims to
be and originates from the stated country of origin. This is very
important because it is well known by experts that for any given essential
oil, there are several origins and the quality varies tremendously.
Sensory
evaluation
The
first steps of essential oil testing usually begins with sensory
evaluation, since this saves wasting precious time and money on more
expensive analytical procedures and can identify inferior oils quickly. The viscosity, colour, clarity and odour of an essential
oil can help to identify a poor quality oil right at the outset - provided you know
precisely what to look for.
For example, a sample of Rose Otto which appears too
mobile at a low temperature is a typical example of an oil which we would
immediately regard as very suspicious. Genuine Rose Otto oil congeals
at around 16 degrees centigrade because of the natural waxes that are
present, therefore if it is still mobile at or below this temperature, it
is likely that the oil has been adulterated or 'extended' in some way.
Similarly, Geranium oil can be purchased in bulk from a wide range of
geographical locations such as Egypt, China, Africa and Reunion at prices
that vary considerably. Since Chinese Geranium oil is green and not a
golden colour like the Egyptian material, it isn't difficult to spot the
difference visually. It is not uncommon for unscrupulous traders to try
and pass off one as the other, since at times of market fluctuations the
purchase prices vary considerably.
Odour evaluation
An
odour test can also help
to determine if an oil is really what it purports to be, since certain
adulterants can be identified in this way. Some essential oils such as
Lavender are available from several geographical locations, and a trained
nose will be able to detect if a so called 'French' Lavender is in fact
from another, less desirable (or less expensive) origin.
Sensory evaluation is a simple but effective method of identifying
clumsily adulterated oils quite quickly, but remains only one of many
procedures that must be conducted during the search for purity. Other, more
sophisticated methods of analysis must be employed before a clearer picture
can be formed of an oils authenticity.
Physical parameters
If an essential oil sample passes all of the sensory tests, the
next stage is to test the physical parameters of the essential oil by
means of measuring the Specific Gravity, Optical Rotation and Refractive Index.
This is a more searching examination that will confirm or reject the authenticity of an
oil’s declared botanical species and country of origin, whilst possibly
revealing any adulteration with a foreign substance.
The combination of these
physical tests is usually sufficient to determine if it is worth proceeding
to the final stage of testing an essential oil. If an oil successfully passes the first two stages it is then
independently tested by a laboratory using Gas Chromatography/Mass
Spectrometry (GC/MS).
Gas chromatography
When
using Gas Chromatography to test an essential oil, a tiny sample of the oil is injected
(pictured right) into the
apparatus which contains a very thin
coiled silica tube called a
‘capillary column’. This capillary column may measure up to 100 metres in
length and is coated on the inside with
a material that has an affinity to different chemicals at different
temperatures. The column is housed within a temperature regulated
oven and is programmed to steadily
increase in temperature over a period of time in a very precise manner.
When the sample of oil is injected into the column it immediately
vaporises, and an inert carrier gas (usually hydrogen or helium) moves the
vapour along the column to a detector called a Flame Ionisation Detector which is
situated at the end of the column.
Component identification
The
flame ioniser detector responds quantitatively to the vaporised
constituents of the oil and converts this information, via an
integrator/computer, into proportional peaks printed onto computer listing
paper. The height of every ‘peak’ on the graph corresponds
proportionally to the level of that component within the oil.
Every
individual component of the essential oil can be identified by the time at
which the peak elutes on the trace. The data produced can then be compared
to an established ‘profile’ or ‘fingerprint’ for that particular
essential oil to finally determine the purity of the oil.
Adulterants
can usually be identified by this means of
testing, although it does require the expertise of an organic analytical
chemist who is a specialist in this area to fully and accurately interpret
the results of the testing. Variations in climatic conditions, and the
type of soil in which a plant was grown will produce natural variations in
essential oils produced from the same species.
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Gas
Chromatograph |

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